Quantigene 2 0 user manual

User manual of the quanta lb6m series layer 2, 3, and ipv6 plus qos managed switch. Quantigene plex represents a promising diagnostic tool for cellof. Quantigene viewrna probe sets are ordered separately. Multiplex transcriptional analysis of paraffinembedded. The quantigene plex assay is hybridizationbased and incorporates branched dna bdna. Elisa assay concentrations of 1 x 106 cells were cultured in 1 ml complete media in 6well plate with 5% co 2, at 37c for 24 hours. Table 2 3 horizontal accuracy statistic conversions 2drms 0. Quantigene plex tutorial for quantitative multiplexed gene expression using the vortemp duration. Briefly, sequential hybridisations were conducted at 55. Clinical diagnostics instruments luminex 100200 system. The standard quantigene protocol panomics, quantigene 2. The startup and shutdown maintenance routines are both part of the daily maintenance schedule. Starting on day 5, half of the plating medium was changed every 4872 hours. A biotek elx 405 select plate washer was used for all of the wash steps in.

Component quantity storage qs0149 10 samples qs0150 25 samples qs0151 100 samples homogenizing solution 20 ml 50 ml 200 ml 1530. For nih swiss mice, a similar dynamic change in plasma b1 dna was seen, except in the linear range from 3 to 7 gy, with r 2 0. Rna was isolated from thawed spleen lysates using the rneasy kit, according to the manufacturers instructions qiagen, valencia, ca. Resequencing array types about this manual resequencing array types resequencing arrays provide an efficient and costeffective method for resequencing large amounts of dna. Quality control parameters on a large dataset of regionally. Quantigene sample assessment kit product information sheet. Quantifiable signals are those signals within the assays linear range. Clear instructions on how to assess the data quality and tools to perform this analysis in high throughput mode are, however, currently lacking. Hypoxanthineguanine phosphoribosyltransferase hprt was used as housekeeping gene. The viewrna ish tissue assay consists of 3 modules, each sold separately. View brochures, application notes, msds, publications, and other resources for quantigene. On day 5 of the 3d culture 2d hepatic cultures were initiated in collagen i coated 24. Quantigene plex assays provide a fast and highthroughput solution for multiplexed gene expression quantitation, allowing the simultaneous measurement of up to 80 genes of interest in a single well of a 96 or 384well plate.

All reagents described below were purchased from affymetrix unless noted. Palm tocotrienols decrease levels of proangiogenic. Each oligonucleotide probe set contains three types of synthetic probes, capture extenders. If the amount of sample is not limiting, use an input that has a signalbackground ratio of at least 3fold. Background is defined as signal from a sample well without sample input. Experiments were performed according to manufacturers instructions. Assay specific reagents size qs0101 cell lysate sample preparation kit1 1 sufficient for preparing bulk lysates from up to approximately 1. Flashtag biotin hsr rna labeling kit flashtag biotin hsr rna labeling kit, 10 rxn pn 901910 or 30 rxn pn 901911.

View brochures, application notes, msds, publications, and other resources for quantigene singleplex assays. Briefly, sequential hybridisations were conducted at 55c for 1. The quantigene plex assay is hybridizationbased and incorporates branched dna bdna technology, which uses signal. The luminex 100200 system is the combination of three core xmap technologies. Sep 18, 2012 quantigene plex tutorial for quantitative multiplexed gene expression using the vortemp duration. It is assumed that all four total rna samples will be provided in aqueous solution ready for use. Lets go over the processes of starting up and shutting down the luminex 200 instrument. Agilent technologies e3632a user manual pdf download. The ability to quantify specific rna molecules within a sample lies in the design of a quantigene 2. Detection and profiling of diarrheic marine biotoxins in. Resequencing array types about this manual resequencing array types resequencing arrays provide an efficient and costeffective method for resequencing large amounts of. On day 5 of the 3d culture 2d hepatic cultures were initiated in collagen i coated 24well plates using the same lots of phh following user manual.

Declaration of conformity this doc applies to abovelisted products placed on the eu market. The estimated time required may be longer if multiple samples. Probe sets were designed and synthesized by affymetrix, inc. This figure was adapted from the quantigene plex 2. A linear regression analysis, performed between doses and plasma b1 dna concentration in the range 36 gy, demonstrated an excellent linearity with r 2 0. Scale according to the number of assays to be run, and include 40% overage. The multiplex bdna assays were run as recommended in the quantigene plex 2. Quantigene singleplex assay thermo fisher scientific. The first is xmap microspheres, a family of fluorescently dyed micronsized polystyrene microspheres that act as both the identifier and the solid surface to build the assay. Transcriptional profiling of tlr478stimulated guinea.

Signal amplification and detection the standard quantigene protocol panomics, quantigene 2. User guide, affymetrix genechip sequence analysis software. These data demonstrate the ability of the elx405 magnetic bead washer to effectively perform the washes necessary for the quantigene plex 2. Working probe set was prepared foreach target, and capturing of target rna was carried out at 55 c overnight. In collaboration with affymetrix, a series of quantigene plex 2. Workflow of the in situ hybridization assay based on bdna technology for a cultured cells and b ffpe. When describing a procedure for publication using this product, please refer to it as the quantigene 2. General the ivis 200 has the capacity to image up to 5 mice or 2 rats simultaneously, ivis 200 basics. Quantigene viewrna a robust and sensitive assay for mrna.

For more information about the components provided in the assay, please refer to the user manual, quantigene viewrna for ffpe samples, available at figure 2. Quantigene 2 0 user manual download file size panasonic dmp bd55 user manual 174 mb bitdefender internet security 20 manual update 350 mb pvm manual user guide 161 mb. User manual of the quanta lb6m series layer 2, 3, and ipv6. Quantigene 2 0 user manual download file size panasonic dmp bd55 user manual 174 mb bitdefender internet security 20. The second is a flow cytometrybased instrument, the luminex 100200 analyzer, which. Page 162 supplemental characteristics supplemental characteristics output programming range maximum programmable values low range 0 to 15. L signal background subtracted mfi observed fold change expected fold change % obsexp 60 3100 3. Agilent 1260 infinity quaternary lc manual and quick reference 3 in this book in this book this book describes the agilent 1260 infinity quaternary lc 1 introduction this chapter gives an introduction to the agilent 1260 infinity quaternary lc, the underlying concepts and the features of the agilent 1260 infinity quaternary lc. The spheroids formed within 5 days of cell seeding. The role of innate immunity in promoting saersmediated. The instructions and safety information in the user.

Quantigene plex gene expression assay thermo fisher. Cell lysates from cultured cells or whole blood tissue homogenates from fresh, frozen, or formalinfixed, paraffinembedded ffpe tissues purified or in vitro transcribed ivt rna quantigene singleplex assay user guide 5. Quantitative gene expression analysis using the quantigene 2. It meets affymetrix plate temperature control requirements when performing the sensitive target hybridization steps. The evalua tion kit contains all necessary materials and reagents for 192 quantigene 2. Using an initial soak time of 60 seconds to capture the beads, wash programs consisting of a series of aspiration and dispense steps each followed with a soak under magnetization were performed. The bdna assay is a hybridizationbased method of targetspecific rna. Pdf im so excited my band is turning up tomorrow in the uk.

Online manual 3,0,0,0 qiagen bioinformatics manuals. This user guide contains instructions for using the quantigene plex assay with the following sample types. Ivtv utilities for hauppauge pvr pci cards update all toshiba satellite l755s5242 drivers in just 2 minutes go to the above link and get the iespyad download. Quantigene sample assessment kit product information. Automated washing of magnetic bead quantigene plex 2. Transcriptional profiling of tlr478stimulated guinea pig. Development of a multiplex gene expression assay for. Policy think tanks and research institutes also produce and publish many policy reports.

Operations guides and other technical documentation. Contents and storage refer to the product label for expiration date. Probe design software was used to generate probe sets for singleplex or multiplex bdna assays. Quantigene singleplex gene expression assay literature. Maqc study data generation plan genospectra quantigene platform. For each rna, the plot displays the yields from eight titration points. Each module below is sold separately in multiple sizes. About 100 ng rnawell was loaded into 96well plate for the detection of target probe, and 1 ng rnawell was loaded for the detection of bactin. Pretreatment assay optimization procedures and typical results in the affymetrix quantigene viewrna ish tissue assay user manual before starting the protocol. Multiplex transcriptional analysis of paraffinembedded liver. This manual provides complete instructions for performing the viewrna ish.

Jan 15, 2018 lets go over the processes of starting up and shutting down the luminex 200 instrument. Evaluation kits enable new users to evaluate the quantigene 2. For more information on quantigene singleplex assays, refer to the appropriate quantigene singleplex reagent system user manual. Quantigene plex analysis of adme gene expression in. After 24 h exposure of caco 2 cells in the 96 well format every exposure performed in triplicate, e. Qiagen genereader user manual for advanced process flow apf instrument configuration 122016 10 2 safety information before using the genereader, it is essential that you read this user manual carefully and pay particular attention to the safety information. Maqc study data generation plan genospectra quantigene. Phos plus and 10% lithium lauryl sulfate was used instead of lumigen aps.